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SAS institute computer generated randomization sequence
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Random Sequence Generation, supplied by AstraZeneca ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Verlag GmbH efficient and simple generation of random simple connected graphs with prescribed degree sequence
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General Biosystems Inc random srna sequence (24 nt)
Double-strand siRNAs (siRNA-1 and siRNA-2) and <t>single-strand</t> <t>inhibitor</t> against the precursor sequence of milR-87, as well as a random <t>small</t> <t>RNA</t> (24 nt) serving as a negative control (NC) were synthesized and transfected into Foc with aid of lipofectamine 2000 (Invitrogen). After 2 days transfection, conidia suspensions of the transfected strains and positive control of the ΔmilR-87 mutant, as well as non-transfected strain of Foc (CK) were inoculated on the banana leaves. After 3 days, the lesions on banana leaves were photographed. ( A ) Symptoms of pathogenicity on the host banana leaves. ( B ) Lesions sizes were measured for accessing virulence of the different strains. Error bars indicate S. D. (n=3). *, p<0.05; **, p<0.01. ( C ) Relative expression of milR-87 in the transfected strains, the non-transfected strain of Foc (CK), and the ΔmilR-87 mutant. The total RNAs of the tested strains cultured at 28°C for 7 days were extracted for milRNA detection by qRT-PCR. Relative expression levels of milR-87 in the transfected strains were normalized to that of non-transfected strain of Foc (CK), which was set as 1. snRNA U4 was used as internal control. Error bars indicate S. D. (n=3). **, p<0.01.
Random Srna Sequence (24 Nt), supplied by General Biosystems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SAS institute computer-generated random allocation sequence
Double-strand siRNAs (siRNA-1 and siRNA-2) and <t>single-strand</t> <t>inhibitor</t> against the precursor sequence of milR-87, as well as a random <t>small</t> <t>RNA</t> (24 nt) serving as a negative control (NC) were synthesized and transfected into Foc with aid of lipofectamine 2000 (Invitrogen). After 2 days transfection, conidia suspensions of the transfected strains and positive control of the ΔmilR-87 mutant, as well as non-transfected strain of Foc (CK) were inoculated on the banana leaves. After 3 days, the lesions on banana leaves were photographed. ( A ) Symptoms of pathogenicity on the host banana leaves. ( B ) Lesions sizes were measured for accessing virulence of the different strains. Error bars indicate S. D. (n=3). *, p<0.05; **, p<0.01. ( C ) Relative expression of milR-87 in the transfected strains, the non-transfected strain of Foc (CK), and the ΔmilR-87 mutant. The total RNAs of the tested strains cultured at 28°C for 7 days were extracted for milRNA detection by qRT-PCR. Relative expression levels of milR-87 in the transfected strains were normalized to that of non-transfected strain of Foc (CK), which was set as 1. snRNA U4 was used as internal control. Error bars indicate S. D. (n=3). **, p<0.01.
Computer Generated Random Allocation Sequence, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GraphPad Software Inc random sequence generator
Double-strand siRNAs (siRNA-1 and siRNA-2) and <t>single-strand</t> <t>inhibitor</t> against the precursor sequence of milR-87, as well as a random <t>small</t> <t>RNA</t> (24 nt) serving as a negative control (NC) were synthesized and transfected into Foc with aid of lipofectamine 2000 (Invitrogen). After 2 days transfection, conidia suspensions of the transfected strains and positive control of the ΔmilR-87 mutant, as well as non-transfected strain of Foc (CK) were inoculated on the banana leaves. After 3 days, the lesions on banana leaves were photographed. ( A ) Symptoms of pathogenicity on the host banana leaves. ( B ) Lesions sizes were measured for accessing virulence of the different strains. Error bars indicate S. D. (n=3). *, p<0.05; **, p<0.01. ( C ) Relative expression of milR-87 in the transfected strains, the non-transfected strain of Foc (CK), and the ΔmilR-87 mutant. The total RNAs of the tested strains cultured at 28°C for 7 days were extracted for milRNA detection by qRT-PCR. Relative expression levels of milR-87 in the transfected strains were normalized to that of non-transfected strain of Foc (CK), which was set as 1. snRNA U4 was used as internal control. Error bars indicate S. D. (n=3). **, p<0.01.
Random Sequence Generator, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bell Medical Solutions Inc computer-generated block random allocation sequence
Double-strand siRNAs (siRNA-1 and siRNA-2) and <t>single-strand</t> <t>inhibitor</t> against the precursor sequence of milR-87, as well as a random <t>small</t> <t>RNA</t> (24 nt) serving as a negative control (NC) were synthesized and transfected into Foc with aid of lipofectamine 2000 (Invitrogen). After 2 days transfection, conidia suspensions of the transfected strains and positive control of the ΔmilR-87 mutant, as well as non-transfected strain of Foc (CK) were inoculated on the banana leaves. After 3 days, the lesions on banana leaves were photographed. ( A ) Symptoms of pathogenicity on the host banana leaves. ( B ) Lesions sizes were measured for accessing virulence of the different strains. Error bars indicate S. D. (n=3). *, p<0.05; **, p<0.01. ( C ) Relative expression of milR-87 in the transfected strains, the non-transfected strain of Foc (CK), and the ΔmilR-87 mutant. The total RNAs of the tested strains cultured at 28°C for 7 days were extracted for milRNA detection by qRT-PCR. Relative expression levels of milR-87 in the transfected strains were normalized to that of non-transfected strain of Foc (CK), which was set as 1. snRNA U4 was used as internal control. Error bars indicate S. D. (n=3). **, p<0.01.
Computer Generated Block Random Allocation Sequence, supplied by Bell Medical Solutions Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Turck Inc non-random sequence generation
Double-strand siRNAs (siRNA-1 and siRNA-2) and <t>single-strand</t> <t>inhibitor</t> against the precursor sequence of milR-87, as well as a random <t>small</t> <t>RNA</t> (24 nt) serving as a negative control (NC) were synthesized and transfected into Foc with aid of lipofectamine 2000 (Invitrogen). After 2 days transfection, conidia suspensions of the transfected strains and positive control of the ΔmilR-87 mutant, as well as non-transfected strain of Foc (CK) were inoculated on the banana leaves. After 3 days, the lesions on banana leaves were photographed. ( A ) Symptoms of pathogenicity on the host banana leaves. ( B ) Lesions sizes were measured for accessing virulence of the different strains. Error bars indicate S. D. (n=3). *, p<0.05; **, p<0.01. ( C ) Relative expression of milR-87 in the transfected strains, the non-transfected strain of Foc (CK), and the ΔmilR-87 mutant. The total RNAs of the tested strains cultured at 28°C for 7 days were extracted for milRNA detection by qRT-PCR. Relative expression levels of milR-87 in the transfected strains were normalized to that of non-transfected strain of Foc (CK), which was set as 1. snRNA U4 was used as internal control. Error bars indicate S. D. (n=3). **, p<0.01.
Non Random Sequence Generation, supplied by Turck Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GraphPad Software Inc computer-generated random number sequence stat matetm version 1.01i
Double-strand siRNAs (siRNA-1 and siRNA-2) and <t>single-strand</t> <t>inhibitor</t> against the precursor sequence of milR-87, as well as a random <t>small</t> <t>RNA</t> (24 nt) serving as a negative control (NC) were synthesized and transfected into Foc with aid of lipofectamine 2000 (Invitrogen). After 2 days transfection, conidia suspensions of the transfected strains and positive control of the ΔmilR-87 mutant, as well as non-transfected strain of Foc (CK) were inoculated on the banana leaves. After 3 days, the lesions on banana leaves were photographed. ( A ) Symptoms of pathogenicity on the host banana leaves. ( B ) Lesions sizes were measured for accessing virulence of the different strains. Error bars indicate S. D. (n=3). *, p<0.05; **, p<0.01. ( C ) Relative expression of milR-87 in the transfected strains, the non-transfected strain of Foc (CK), and the ΔmilR-87 mutant. The total RNAs of the tested strains cultured at 28°C for 7 days were extracted for milRNA detection by qRT-PCR. Relative expression levels of milR-87 in the transfected strains were normalized to that of non-transfected strain of Foc (CK), which was set as 1. snRNA U4 was used as internal control. Error bars indicate S. D. (n=3). **, p<0.01.
Computer Generated Random Number Sequence Stat Matetm Version 1.01i, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Double-strand siRNAs (siRNA-1 and siRNA-2) and single-strand inhibitor against the precursor sequence of milR-87, as well as a random small RNA (24 nt) serving as a negative control (NC) were synthesized and transfected into Foc with aid of lipofectamine 2000 (Invitrogen). After 2 days transfection, conidia suspensions of the transfected strains and positive control of the ΔmilR-87 mutant, as well as non-transfected strain of Foc (CK) were inoculated on the banana leaves. After 3 days, the lesions on banana leaves were photographed. ( A ) Symptoms of pathogenicity on the host banana leaves. ( B ) Lesions sizes were measured for accessing virulence of the different strains. Error bars indicate S. D. (n=3). *, p<0.05; **, p<0.01. ( C ) Relative expression of milR-87 in the transfected strains, the non-transfected strain of Foc (CK), and the ΔmilR-87 mutant. The total RNAs of the tested strains cultured at 28°C for 7 days were extracted for milRNA detection by qRT-PCR. Relative expression levels of milR-87 in the transfected strains were normalized to that of non-transfected strain of Foc (CK), which was set as 1. snRNA U4 was used as internal control. Error bars indicate S. D. (n=3). **, p<0.01.

Journal: bioRxiv

Article Title: FoQDE2-dependent milRNA promotes Fusarium oxysporum f. sp. cubense virulence by targeting a glycosyl hydrolase coding gene at transcriptional level

doi: 10.1101/2021.12.02.470887

Figure Lengend Snippet: Double-strand siRNAs (siRNA-1 and siRNA-2) and single-strand inhibitor against the precursor sequence of milR-87, as well as a random small RNA (24 nt) serving as a negative control (NC) were synthesized and transfected into Foc with aid of lipofectamine 2000 (Invitrogen). After 2 days transfection, conidia suspensions of the transfected strains and positive control of the ΔmilR-87 mutant, as well as non-transfected strain of Foc (CK) were inoculated on the banana leaves. After 3 days, the lesions on banana leaves were photographed. ( A ) Symptoms of pathogenicity on the host banana leaves. ( B ) Lesions sizes were measured for accessing virulence of the different strains. Error bars indicate S. D. (n=3). *, p<0.05; **, p<0.01. ( C ) Relative expression of milR-87 in the transfected strains, the non-transfected strain of Foc (CK), and the ΔmilR-87 mutant. The total RNAs of the tested strains cultured at 28°C for 7 days were extracted for milRNA detection by qRT-PCR. Relative expression levels of milR-87 in the transfected strains were normalized to that of non-transfected strain of Foc (CK), which was set as 1. snRNA U4 was used as internal control. Error bars indicate S. D. (n=3). **, p<0.01.

Article Snippet: Double-strand siRNAs (siRNA-1 and siRNA-2) and single-strand inhibitor against the precursor sequence of milR-87, as well as a random sRNA sequence (24 nt) serving as a negative control (NC) were designed and synthesized by General Biosystems company.

Techniques: Sequencing, Negative Control, Synthesized, Transfection, Positive Control, Mutagenesis, Expressing, Cell Culture, Quantitative RT-PCR, Control